| Participating Team National History |
| Age | Team | Championship Results |
| U20F | Chicago Magic Blue | US Club 2024 Regional Competitor
|
| Cincinnati Soccer Alliance | USYS 2025 Ohio State Semi-Finalist
USYS 2025 Ohio State Semi-Finalist
|
| Kentucky Fire Juniors Premier | USYS 2024 Kentucky State Finalist
|
| Javanon 97 Girls Black | USYS 2025 Regional Competitor
|
| GCUFC 97 GIRLS PREMIER | USYS 2024 Regional Semi-Finalist
|
| FC Pride 97 Elite White | USYS 2025 Indiana State Finalist
USYS 2025 Indiana State Finalist
|
| SSA Chelsea 97G Elite | USYS 2024 Georgia State Finalist
|
| Iowa City Alliance Speed | USYS 2024 Regional Competitor
|
| WC United G97 Elite | USYS 2016 Ohio State Semi-Finalist
|
| TFC 98 United | USYS 2025 Regional Competitor
USYS 2025 Regional Competitor
|
| FC Pride 98 Elite Girls | USYS 2024 Indiana State Finalist
|
| Alliance FC IN 99G | USYS 2025 Indiana State Semi-Finalist
|
| Andromeda FC 99G West Red | USYS 2016 Texas State Semi-Finalist
USYS 2025 Texas State Semi-Finalist
|
| Club Ohio Cincinnati | USYS 2025 Ohio State Semi-Finalist
USYS 2025 Ohio State Semi-Finalist
|
| Derby City Rovers 99/98 | USYS 2025 Kentucky State Semi-Finalist
USYS 2024 Kentucky State Semi-Finalist
|
| GCUFC 99 GIRLS | USYS 2025 Mississippi State Champion
|
| MS Rush 99G Premier | USYS 2025 Mississippi State Finalist
USYS 2024 Regional Competitor
USYS 2025 Mississippi State Finalist
|
| Warren County United G98 Elite | USYS 2016 Ohio State Semi-Finalist
USYS 2016 Ohio State Semi-Finalist
|
| FC Pride 99 Elite Girls | USYS 2016 Indiana State Finalist
|
| FC Pride 00 Elite | USYS 2017 Indiana State Champion
USYS 2017 Regional Competitor
|
| MSC Lady Strikers 2000 Premier | US Club 2016 Regional Competitor
US Club 2016 Regional Competitor
|
| United 1996 FC 00 Intl | USYS 2018 Regional Competitor
|
| AFC Lightning '00 Girls Elite | USYS 2019 Georgia State Semi-Finalist
|
| HFC Girls 00 Blue | USYS 2018 Alabama State Semi-Finalist
|
| SSA Chelsea 00G Elite | USYS 2025 Georgia State Semi-Finalist
|
| ZYSA 00 Girls Select | USYS 2016 Indiana State Champion
USYS 2016 Indiana State Champion
USYS 2024 Indiana State Finalist
|
| JB Marine Elite | US Club 2016 Regional Competitor
|
| ASA 2000 Girls Gold | USYS 2025 Tennessee State Finalist
|
| 00 Lady Lobos Rush Premier | USYS 2018 Regional Competitor
|