| BSC 04 Boys White | Bluegrass SC | KY |
| FC Alliance 04B ENPL | FC Alliance (TN) | TN |
| FC Alliance 04B TSL2 | FC Alliance (TN) | TN |
| FC Alliance 05B ENPL | FC Alliance (TN) | TN |
| HFC 04B White | Highland Football Club (HFC) | NC |
| Metro FC 04B Rapids | Metro FC (OH) | OH |
| Ohio Galaxies FC 04 Black | Ohio Galaxies FC | OH |
| FC Alliance 03 Boys ENPL | FC Alliance (TN) | TN |
| FC Alliance 03 Boys TSL2 | FC Alliance (TN) | TN |
| HFC 03 White | Highland Football Club (HFC) | NC |
| Metro FC B03 Rapids | Metro FC (OH) | OH |
| Ohio Galaxies FC 03 Red | Ohio Galaxies FC | OH |
| Ohio Galaxies FC 03 White | Ohio Galaxies FC | OH |
| TSC 03B Showcase | Tennessee Soccer Club (TN) | TN |
| CFFC 02B | Camp Forest Football Club | TN |
| FC Alliance 02 Boys ENPL | FC Alliance (TN) | TN |
| Metro FC 02B Rapids | Metro FC (OH) | OH |
| Ohio Galaxies FC 02 Red | Ohio Galaxies FC | OH |
| One7 02B | One7 Academy | NC |
| Bluegrass SC 02 Boys | Bluegrass SC | KY |
| Commonwealth SC 2002 Boys Gold | Commonwealth Soccer Club | KY |
| FC Alliance 02 Boys Red | FC Alliance (TN) | TN |
| HFC 02B White | Highland Football Club (HFC) | NC |
| LFC 02B Red | Lexington FC | KY |
| LNSC Force Black | Lake Norman Soccer Club (LNSC) | NC |
| DUFC WARRIORS | Denver United FC | NC |
| FC Alliance 00/01 B ENPL | FC Alliance (TN) | TN |
| Metro FC 01B North | Metro FC (OH) | OH |
| One7 Academy 00b | One7 Academy | NC |
| United 1996 FC International 2000 | United 1996 | KY |
| Wcsa 2001b Elite | Warren County SA | OH |
| Lake Norman SC 01 Blue Force | Lake Norman Soccer Club (LNSC) | NC |
| Magic Waterford 01 | Magic Soccer Club (MI) | MI |
| NTH NASA 00B Premier | NASA Tophat (NTH) | GA |
| USF Real 00 Boys Premier | USF Real | IN |
| Atletico Flames U19 White | Atletico Flames Futbol Club | KY |
| Lexington FC 00/01 White | Lexington FC | KY |
| SWVA RUSH 00B NERO | SWVA Rush | VA |
| TSC Black Attack | Trojans Soccer Club | OH |