| DFA 07 Elite | Dayton Futbol Academy | OH |
| FC Alliance 07B ECNL-RL | FC Alliance (TN) | TN |
| Flames U15 Boys | Atletico Flames Futbol Club | KY |
| Indiana Fire Juniors South 07b Red | Indiana Fire Juniors | IN |
| Indiana Impact SC 07 Boys Elite 1 | Indiana Impact SC | IN |
| Ohio Galaxies FC 07 Red | Ohio Galaxies FC | OH |
| 06 (16U) HFC White | Highland Football Club (HFC) | NC |
| 2006 Premier Boys | IMG Soccer Academy (FL) | FL |
| Aris FC 06B | Aris FC | TN |
| Chattanooga FC 06 Navy | Chattanooga FC Academy | TN |
| Commonwealth SC Csc 06 Boys Gold | Commonwealth Soccer Club | KY |
| DFA 2006 Elite Boys | Dayton Futbol Academy (DFA) | OH |
| FC Alliance 06B ECNL-RL | FC Alliance (TN) | TN |
| Hoosier FC 2006 Elite Wolves | Hoosier Futbol Club | IN |
| IFJ South 06B Premier Red | Indiana Fire Juniors | IN |
| Impact 06 Boys Elite1 | Indiana Impact SC | IN |
| JSC 2006 Boys | Jackson Soccer Club | TN |
| Nationals Genesee 06B Red | Nationals Soccer Club | MI |
| TSC 06B Williamson Elite | Tennessee Soccer Club (TN) | TN |
| 05 (17U) CVYSA Blast Blue | Catawba Valley YSA (CVYSA) | NC |
| 05 (17U) HFC White | Highland Football Club (HFC) | NC |
| 2005 Elite (DFA) | Dayton Futbol Academy (DFA) | OH |
| 2005 Premier Boys | IMG Soccer Academy (FL) | FL |
| Commonwealth SC Csc 05 Boys Gold | Commonwealth Soccer Club | KY |
| FC Alliance 05B ECNL-RL | FC Alliance (TN) | TN |
| Impact 05 Boys Elite | Indiana Impact SC | IN |
| Indiana Fire Juniors South 05b Premier Red | Indiana Fire Juniors | IN |
| LFC 05B Red | Lexington FC | KY |
| Rossoneri 05 Boys | Derby City Rossoneri | KY |
| TN Select 05 D1 Boys | Tennessee Select Soccer Academy | TN |
| TSC Williamson Premier 05 Boys | Tennessee Soccer Club (TN) | TN |
| 04 (18U) HFC White | Highland Football Club (HFC) | NC |
| Atletico Flames 04B Maroon | Atletico Flames Futbol Club | KY |
| Chattanooga FC 04 White | Chattanooga FC Academy | TN |
| DFA 04 Boys Premier | Dayton Futbol Academy (DFA) | OH |
| Genesee 04 black | Nationals | MI |
| Impact 04 Boys Elite | Indiana Impact SC | IN |
| JSC 2004 Showcase | Jackson Soccer Club | TN |
| NUSA 05B Maroon | Nashville United Soccer Acd (NUSA) | TN |
| TN Select 03 Boys | Tennessee Select Soccer Academy | TN |
| FC Alliance 03B ECNL-RL | FC Alliance (TN) | TN |
| FC Alliance 03B Showcase | FC Alliance (TN) | TN |
| Genesee 03 black | Nationals | MI |
| IMG Premier Boys Blue | IMG Soccer Academy (FL) | FL |
| IMG Premier Boys White | IMG Soccer Academy (FL) | FL |
| Indiana Fire Juniors South 03/04B | Indiana Fire Juniors South | IN |
| KY Rush 03 East | Kentucky Rush Soccer Club | KY |
| Ohio Galaxies FC 03/04 Black | Ohio Galaxies FC | OH |