| 2011 CISC South York Blue | Charlotte Independence (CISC) | SC |
| FC Alliance 2011B East | FC Alliance (TN) | TN |
| FC Alliance 2012 Boys Central | FC Alliance (TN) | TN |
| OESA-KY 11B Academy | Ohio Elite Soccer Academy-Kentucky | KY |
| FC Alliance 2010 West | FC Alliance (TN) | TN |
| JCFC 2010B | Johnson City Futbol Club | TN |
| MSC Strikers 2010B Grey | Murfreesboro Soccer Club | TN |
| OESA-KY 10B Academy | Ohio Elite Soccer Academy-Kentucky | KY |
| FC Alliance 2010 Boys East | FC Alliance (TN) | TN |
| LFC 10B Blue | Lexington FC | KY |
| LSA 10 White | Lanier Soccer Association | GA |
| MSC Strikers 2010B White | Murfreesboro Soccer Club | TN |
| OESA-KY 10B Gold | Ohio Elite Soccer Academy-Kentucky | KY |
| USA North 10/11B Select | USA North | TN |
| Atlanta Fire 2009 Gold | Atlanta Fire United | GA |
| FC Alliance 2009 Boys Black 2 | FC Alliance (TN) | TN |
| Nelson Elite Boys 09 | Nelson County YSA - Elite | KY |
| NWC Alliance 09 Boys | NWC Alliance | OH |
| TUSC 09B Red | Tennessee United Soccer Club | TN |
| Club Ohio Dayton 09 | Club Ohio Soccer | OH |
| JCFC 2009 Boys | Johnson City Futbol Club | TN |
| KFC Crush 10B Bayern Munich | Knoxville Football Club (TN) | TN |
| Mercury Red 09 B | Mercury SC | OH |
| Monroe Premier U11 Boys Hornets Johnson | Monroe Premier FC | OH |
| OESA-KY 09B Academy | Ohio Elite Soccer Academy-Kentucky | KY |
| Redoubt 09 Generals | Redoubt Soccer | TN |
| Club Ohio Dayton 08 | Club Ohio Soccer | OH |
| Cuse 2008 Barcelona | Cincinnati United Southeast (CUSE) | OH |
| OESA-KY 08B Academy | Ohio Elite Soccer Academy-Kentucky | KY |
| YCVSC Eagles Orange | YCVSC Eagles Soccer Club | NC |
| Knoxville FC Crush 07B Valencia Premier | Knoxville Football Club (TN) | TN |
| NWC Alliance 07 Boys | NWC Alliance | OH |
| OESA-KY 07B Academy | Ohio Elite Soccer Academy-Kentucky | KY |
| TUSC 08B Red | Tennessee United Soccer Club | TN |
| CKY Storm 07 Boys White | Central Kentucky Storm SC | KY |
| Club Ohio Dayton - Green | Club Ohio Soccer | OH |
| FOSC B07 Elite | Fairfield Optimist Soccer Club (OH) | OH |
| Knoxville FC Crush 07B Valencia Elite | Knoxville Football Club (TN) | TN |
| Knoxville Reds 08B Celtic | Knoxville Reds FC | TN |
| Central KY Storm 07 Boys Black Team | Central Kentucky Storm SC | KY |
| Cincinnati United Cuse Arsenal 07 | Cincinnati United SouthEast (CUSE) | OH |
| NWC Alliance 08 Boys | NWC Alliance | OH |
| TFA 08B Premier | Total Futbol Academy (OH) | OH |
| ANPSL PSL/HURRICANES, INC PSL HURRICANES 2006 | Port St. Lucie Soccer Club (PSL) | FL |
| NWC Alliance 06 Boys | NWC Alliance | OH |
| OESA-KY 06 Academy | Ohio Elite Soccer Academy-Kentucky | KY |
| TUSC 06B Red | Tennessee United Soccer Club | TN |
| BA CLT U15 Boys Blue | Barca Academy Charlotte | NC |
| BA CLT U15 Boys Garnet | Barca Academy Charlotte | NC |
| CUSE B05 Elite | Cincinnati United SouthEast (CUSE) | OH |
| FC Alliance 06/05-TSL2 | FC Alliance (TN) | TN |
| Nelson Elite 05 Boys | Nelson County YSA - Elite | KY |
| BA CLT U16 Boys Garnet | Barca Academy Charlotte | NC |
| CUSE 04B Elite | Cincinnati United SouthEast (CUSE) | OH |
| FC Kentucky 04B | FC Kentucky | KY |
| LFC 04B Blue | Lexington FC | KY |
| Commonwealth SC 2003 Boys Gold | Commonwealth Soccer Club | KY |
| Fc Kentucky | FC Kentucky | KY |
| Georgetown FC Gfc 01 Boys | Georgetown Football Club | KY |
| KY Rush Boys | Kentucky Rush Soccer Club | KY |
| Lakota FC B03/04 Black | Lakota FC | OH |
| Nelson Elite - 01 Boys | Nelson County YSA - Elite | KY |
| SEI United 2001 Boys | Southeast Indiana (SEI) United | IN |