| Participating Team National History |
| Age | Team | Championship Results |
| U20F | Ohio Premier OP 02/03 Ecnl RL | US Club 2019 Ohio State Champion
|
| Soccer Vision Academy Fca Vision 03/02 G | US Club 2019 Ohio State Finalist
|
| Lexington FC Lfc 02 Girls White | USYS 2017 Kentucky State Finalist
|
| Real Colorado 2002 Sporting (formerly Na | USYS 2020 Colorado State Finalist
|
| Keystone FC Premier 03g | USYS 2019 Pennsylvania State Finalist
|
| Century United 03G Gold | USYS 2018 Pennsylvania State Semi-Finalist
|
| Hotspurs FC Hotspurs - 2003 Blue | USYS 2017 Pennsylvania State Semi-Finalist
|
| Total Futbol Academy Tfa G03 Elite | USYS 2018 Regional Competitor
|
| Lexington FC 03 Girls White | USYS 2018 Regional Competitor
|
| Nwi Lions United 04g Yellow | USYS 2018 Indiana State Finalist
|
| Hoosier FC 2004 Elite Wolves | USYS 2023 Indiana State Champion
|
| Chicago Empire FC Gold | USYS 2020 Illinois State Quarter-Finalist
|
| Salvo SC 04g Navy | USYS 2023 Regional Competitor
|
| Total Futbol Academy Tfa G04 Elite | USYS 2018 Ohio State Semi-Finalist
|
| Ohio Galaxies FC 04 Elite | USYS 2023 Regional Competitor
|
| Cincinnati United Cup 04g Gold | USYS 2018 Ohio State Champion
USYS 2017 Regional Finalist
|
| Eastern Elite 05 Girls | USYS 2024 Regional Competitor
|
| Club Ohio 2005g Green 1 | USYS 2024 Ohio State Champion
|
| Sporting Queen City Sporting QC G05 I | USYS 2023 Regional Competitor
|
| Cusa G05 Elite | USYS 2024 Ohio State Semi-Finalist
|
| FC Alliance 2005 Girls | USYS 2018 Regional Finalist
|
| North Shore United Nsu 2005 G Blue | USYS 2023 Regional Competitor
|
| United Elite FC 05G | USYS 2023 Indiana State Finalist
|
| Chicago Inter Soccer 2005 Red | USYS 2020 Illinois State Champion
|
| U19F | Mercury G06 Red | USYS 2024 Ohio State Semi-Finalist
|
| Cusa G06 Elite | USYS 2023 Regional Competitor
|
| Ohio Premier OP 06 Ecnl RL | US Club 2019 Ohio State Champion
|
| Chicago Inter Soccer 2006 Red | USYS 2023 Regional Competitor
|
| Glsa Libertyville 06 FC 1974 Elite | USYS 2021 Regional Finalist
|
| Kings Hammer Academy Kha Red | USYS 2023 Regional Competitor
|
| Chattanooga Red Wolves 06g SL | USYS 2021 Tennessee State Semi-Finalist
|
| Keystone FC Premier 06g | USYS 2019 Pennsylvania State Champion
|
| Club Ohio 2006g Green 1 | USYS 2023 Regional Finalist
|
| U18F | Warren County Wcsa 2007g Elite | USYS 2024 Ohio State Finalist
|
| Chattanooga Red Wolves Academy Chattanoo | USYS 2023 Regional Competitor
|
| Club Ohio Dayton G07 Green | USYS 2023 Regional Competitor
|