| Participating Team National History |
| Age | Team | Championship Results |
| U19M | FBSL Tuzos Academy 06B VC | USYS 2024 National Competitor
|
| Miami Breakers FC 2006B Black | USYS 2024 Florida State Champion
|
| U20M | Raiders FC 2005B | USYS 2023 Regional Competitor
|
| Galaxy 2005B CvC | USYS 2024 Regional Competitor
|
| U19M | GLSA Libertyville FC 1974 B2006 Elite | USYS 2024 Regional Competitor
|
| U20M | IFA 2005/06B NL Pro | USYS 2024 Indiana State Champion
|
| Spire FC 2005/06B | USYS 2024 Ohio State Finalist
|
| U19F | Chattanooga Red Wolves 06G | USYS 2021 Tennessee State Semi-Finalist
|
| U20F | NOSC Blast 05G Sahara | USYS 2024 Regional Competitor
|
| U19F | Boreal FC 2006G Green Matesi | USYS 2023 Regional Competitor
|
| U20F | FW United 2005/06G Elite 64 | USYS 2024 Indiana State Champion
|
| U19F | MI Rangers FC 06G Elite | USYS 2023 Regional Semi-Finalist
|
| U19M | CFYSC 2006B Premier | USYS 2023 Regional Semi-Finalist
|
| Strikers Miami FC 2006B Elite | USYS 2024 Florida State Finalist
|
| Kansas Rush 2006B Academy | USYS 2024 Kansas State Champion
|
| U19F | 06 (19U) NCFCY Elite G | USYS 2025 North Carolina State Champion
|
| U18M | BVBIA TX B2007 RL Yellow | USYS 2023 Regional Semi-Finalist
|
| La Roca UT 07B J Milla SC | USYS 2023 Regional Competitor
|
| FC United - DSM 2007B | USYS 2024 Regional Competitor
|
| Toca FC B2007 Elite 64 | USYS 2024 Kansas State Champion
|
| HFC 2007B Elite Wolves NL | USYS 2024 Indiana State Champion
|
| U18F | BVB IA LA 07G Yellow Elite | USYS 2023 Regional Finalist
|
| Club Ohio Dayton 07G Elite | USYS 2023 Regional Competitor
|
| AFA Fillies 2007G Ruthless | USYS 2023 Regional Competitor
|
| Utah Surf 07G B Hale | USYS 2023 Regional Competitor
|
| Solar South 07G FDL G.Adames | USYS 2023 Regional Competitor
|
| 07 LLR Elite 64 | USYS 2023 Regional Competitor
|
| U17M | WA Rush B2008 NL | USYS 2024 Regional Competitor
|
| Utah Celtic FC 2008B | USYS 2023 Regional Competitor
|
| KS Rush B2008 Academy NL | USYS 2024 Kansas State Champion
|
| Toca FC 2008B MLS | USYS 2024 Kansas State Semi-Finalist
|
| Sporting BV 08B Academy | USYS 2024 Kansas State Finalist
|
| Strikers FC North B2008 E64 | USYS 2024 Regional Semi-Finalist
|
| U17F | Team Boca-SABR G2008 Elite 64 | USYS 2023 Regional Competitor
|
| FT Wayne United 2008G | USYS 2024 Indiana State Champion
|
| Michigan Tigers FC 2008G E64 | USYS 2024 Regional Competitor
|
| Ambassadors FC 08G | USYS 2024 Ohio State Champion
|
| St. Croix 08G Academy NL | USYS 2023 Regional Semi-Finalist
|
| Summit FC G2008 JM DC | USYS 2023 Regional Competitor
|
| Chattanooga Red Wolves 08G | USYS 2023 Regional Finalist
|
| DKSC 08G FDL Talavera | USYS 2023 Regional Competitor
|
| PCU 08G Red | USYS 2023 Regional Competitor
|
| U16M | AFC 09B Black | USYS 2024 National Champion
|
| LVSA 2009B Pre-MLS Next | USYS 2021 Nevada State Finalist
|
| Club Ohio 09B National | USYS 2024 Ohio State Champion
|
| KC Legends 2009B Academy | USYS 2024 Kansas State Semi-Finalist
|
| Raiders FC 2009B | USYS 2023 Regional Competitor
|
| U16F | Clermont FC MIFC 2009G | USYS 2024 Regional Competitor
|
| Michigan Tigers FC 2009 Gold | USYS 2023 Regional Competitor
|
| Club Ohio Dayton 09G Elite | USYS 2024 Ohio State Finalist
|
| RSC 2009G Premier | USYS 2023 Regional Competitor
|
| CSA 09G Elite 64 | USYS 2025 North Carolina State Champion
|
| Las Vegas Heat Surf 09G Elite 64 | USYS 2021 Nevada State Champion
|
| Kononia 2009 Lady Fire | USYS 2023 Regional Competitor
|
| EBU 2009G Elite | USYS 2024 Regional Competitor
|
| U15M | Toca FC 2010B MLS | USYS 2024 Kansas State Champion
|
| ZYSA 10B Green | USYS 2024 Indiana State Champion
|
| Oxnard FC 2010B | USYS 2024 Regional Competitor
|
| Ponys FC 2010B | USYS 2024 Regional Champion
|
| BA Pro Miami 2010B De Jong | USYS 2024 Florida State Finalist
|
| Indialantic 2010B Force Black | USYS 2024 Florida State Semi-Finalist
|
| U15F | AR Rising 10G Burgess | USYS 2024 Regional Competitor
|
| KC Legends 2010G Academy Red | USYS 2024 Kansas State Champion
|
| Galaxy SC G2010 Elite 64 | USYS 2023 Regional Semi-Finalist
|
| Nationals Union 10G Black | USYS 2023 Regional Semi-Finalist
|
| Columbus United G2010 Lions | USYS 2024 Ohio State Champion
|
| 10 LLR Elite 64 | USYS 2023 Regional Quarter-Finalist
|
| U16F | Solar South 10G FDL Adames | USYS 2023 Regional Competitor
|
| U15F | North Shore United 2010G Blue | USYS 2024 Regional Competitor
|
| EBU 2010G Elite | USYS 2023 Regional Competitor
|
| U14M | RC Rangers SGA 11B Premier | USYS 2024 Regional Competitor
|
| Illinois Elite BLFC Premiership 11B | USYS 2024 National Finalist
|
| U14F | Columbus United G2011 Lions | USYS 2024 Ohio State Champion
|