| Augusta FC | GA | OVER 40 |
| Birra FC | FL | OVER 40 |
| Borocelona | TN | OVER 40 |
| BREWSERS | NC | OVER 40 |
| Carolina Colortones | NC | OVER 40 |
| Carolina Cosmic FC 40 | NC | OVER 40 |
| CML Hazelwood Brewing | SC | OVER 40 |
| Columbus United | GA | OVER 40 |
| Cosmos | GA | OVER 40 |
| Eagles | GA | OVER 40 |
| FC Raging Booners | NC | OVER 40 |
| Gainesville FC | GA | OVER 40 |
| Greenville United 40 | SC | OVER 40 |
| Highlander FC 40 | GA | OVER 40 |
| Jupiter Strikers FC | FL | OVER 40 |
| Louisville Veterans SC 40 | KY | OVER 40 |
| Maryland Elite FC | MD | OVER 40 |
| Mavericks | NC | OVER 40 |
| Naples OG | FL | OVER 40 |
| Nashville Rogues FC | TN | OVER 40 |
| Printshop Beer | TN | OVER 40 |
| ReUnited Quest | GA | OVER 40 |
| Roswell Rovers | GA | OVER 40 |
| Schurke | AL | OVER 40 |
| Smush FC | NC | OVER 40 |
| Three Notch'd Brewing FC | VA | OVER 40 |
| Triad United FC | NC | OVER 40 |
| United 4Fuchsake | NC | OVER 40 |
| Valhalla Vikings | NC | OVER 40 |
| Vandals FC | TN | OVER 40 |
| Wanderers FC 40 | NC | OVER 40 |
| Wolves | GA | OVER 40 |
| Asheville Fusions | NC | OVER 50 |
| Carolina Colortones 50 | NC | OVER 50 |
| Charlotte Elite | NC | OVER 50 |
| Charlotte Silver Elite 55 | NC | OVER 50 |
| Cincinnati HHC 50 | OH | OVER 50 |
| CML Pink Panthers | SC | OVER 50 |
| Columbus GA FC | GA | OVER 50 |
| FINN FC (NC) | NC | OVER 50 |
| Greenville United 50 | SC | OVER 50 |
| LASC "A" | IN | OVER 50 |
| LASC "B" | KY | OVER 50 |
| Lusitano Rovers ATL | GA | OVER 50 |
| Madra Rua CML | SC | OVER 50 |
| Magic FC | GA | OVER 50 |
| Maryland Lions FC | MD | OVER 50 |
| Maryland Soccer Legends FC Over 50 | MD | OVER 50 |
| Memphis Blues | TN | OVER 50 |
| Rowdies | TN | OVER 50 |
| Silver Strikers | FL | OVER 50 |
| Sugarland Fanatic | TN | OVER 50 |
| TOCA-Highlanders FC | GA | OVER 50 |
| Vintage | GA | OVER 50 |
| VOP Charleston | SC | OVER 50 |
| Wanderers FC 50 | NC | OVER 50 |
| AIS Sunday FC | GA | OVER 58 |
| Boston United | MA | OVER 58 |
| Charleston VOP 60 | SC | OVER 58 |
| Cinci HCC | OH | OVER 58 |
| CML Confluence | SC | OVER 58 |
| Hammers | GA | OVER 58 |
| Indy United LC | IN | OVER 58 |
| International Curmudgeons FC | MD | OVER 58 |
| Nashville Blues 58 | TN | OVER 58 |
| Real Lyold | NC | OVER 58 |
| Soda City | SC | OVER 58 |
| Bobbay's Jorts | GA | COED |
| Booters | GA | COED |
| Carolina Cosmic FC | NC | COED |
| Crown Royal FC | SC | COED |
| Easy Riders | NC | COED |
| EC Oldboys | NC | COED |
| Fulham | TN | COED |
| Hot Spuds | TN | COED |
| Hotshots | TN | COED |
| Jabronies | GA | COED |
| La Real Q.C. FC | NC | COED |
| Nash Azul SC | TN | COED |
| Olde London Road Trash Cats | NC | COED |
| Speedy Turtles ATL | GA | COED |
| SWFL GoalTrotters | FL | COED |
| WU Eagles | SC | COED |