| CUP G05 South 1 | Cincinnati United Soccer Club | OH |
| DPA Cobras 05 Girls Showcase | Dayton Players Academy | OH |
| FC Alliance 05 ECNL | FC Alliance (TN) | TN |
| Georgia Alliance FC '05G EDP | Georgia Alliance FC | GA |
| Barca Academy CLB Martens 05 | Barca Academy Columbus | OH |
| CUP 05G North I | Cincinnati United Soccer Club | OH |
| Eastern Elite 05 Girls | Eastern Elite Soccer Accademy | KY |
| HFC RED G | Highland Football Club (HFC) | NC |
| Cincinnati United Cup 05 South II | Cincinnati United Soccer Club | OH |
| CVYSA BLAST BLUE G | Catawba Valley YSA (CVYSA) | NC |
| KHB 05G Premier | Kings Hammer Bluegrass | KY |
| LFC 05G Red | Lexington FC | KY |
| 04 NCF Elite G | NC Fusion | NC |
| 04 NCF PREMIER G | NC Fusion | NC |
| FC Alliance 04 ECNL | FC Alliance (TN) | TN |
| Fire & Ice Fisa 2004 Petroski | Fire & Ice Soccer Academy | IL |
| HFC RED G | Highland Football Club (HFC) | NC |
| Knoxville Reds 04G United | Knoxville Reds FC | TN |
| Barca Academy CLB Martens 04 | Barca Academy Columbus | OH |
| Concorde Fire 04G SCCL Premier | Concorde Fire Soccer Club | GA |
| CSC 04 Girls Maroon | Clarksville Soccer Club (TN) | TN |
| Tri-Cities United 04/05 Girls Premier | Tri-Cities United Soccer Club | TN |
| CUP 04G Silver | Cincinnati United Soccer Club | OH |
| KHB 04G Premier | Kings Hammer Bluegrass | KY |
| TSC 04G Premier | Tennessee Soccer Club (TN) | TN |
| Warrior 04 Premier | Warrior Soccer Club (OH) | OH |
| 03 NCF ELITE G | NC Fusion | NC |
| FC Alliance 03G ECNL | FC Alliance (TN) | TN |
| Georgia Alliance FC 2003G EDP Blue | Georgia Alliance FC | GA |
| SAFC Ladies 03 | Spartanburg Arsenal FC | SC |
| Georgia Alliance FC 2003G EDP Red | Georgia Alliance FC | GA |
| Metro FC 03G Rapids | Metro FC (OH) | OH |
| MSC 03 Lady Strikers | Murfreesboro Soccer Club | TN |
| NC Fusion 03 G Premier | NC Fusion | NC |
| CESA ANDERSON 03G Challenge | Carolina Elite SA (CESA) | SC |
| CUP 03/04 | Cincinnati United Soccer Club | OH |
| NUSA 03 Girls Maroon | Nashville United Soccer Acd (NUSA) | TN |
| TSC 03 Girls Premier | Tennessee Soccer Club (TN) | TN |
| WCSA G03 Premier | Warren County SA | OH |
| BUSC 03 Lady Hammers | Blount United Soccer Club | TN |
| Commonwealth SC 2003 Girls Gold | Commonwealth Soccer Club | KY |
| Nomads 2003/2004 | Nomads United Soccer Club | TN |
| USA Southeast 03 | USA Southeast | TN |
| 02 NCF ELITE G | NC Fusion | NC |
| CMSC GREEN G | Carolina Mountains Soccer (CMSC) | NC |
| CUSE Elite G02 | Cincinnati United SouthEast (CUSE) | OH |
| FC ALLIANCE 02 ECNL | FC Alliance (TN) | TN |
| Rome Arsenal 02 Red | YMCA Arsenal Rome Soccer Club | GA |
| WCSA 2002G Elite | Warren County SA | OH |
| Butler United 02 Extreme | Butler United Soccer Club | OH |
| Hoosier FC 2002 Elite Wolves Black | Hoosier Futbol Club | IN |
| KHB 02G Premier | Kings Hammer Bluegrass | KY |
| Fire & Ice 2002 Kuehn | Fire & Ice Soccer Academy | IL |
| GSA - '03 Lady Galaxy | Greeneville Soccer Association | TN |
| HFC RED II G | Highland Football Club (HFC) | NC |
| Hoosier FC 2002 Elite Wolves Orange | Hoosier Futbol Club | IN |
| Barca Academy CLB Martens 01-02 | Barca Academy Columbus | OH |
| FC Alliance U19 ECNL Composite | FC Alliance (TN) | TN |
| Fire & Ice 2001 Eversmeyer | Fire & Ice Soccer Academy | IL |
| Tri-Cities United 01/02 Girls Premier | Tri-Cities United Soccer Club | TN |
| UK Soccer - Park Athletic FC | UK Soccer Academy | KY |
| 01 HFC Red G | Highland Football Club (HFC) | NC |
| CVYSA BLAST BLUE G | Catawba Valley YSA (CVYSA) | NC |
| Eastern Elite Soccer Academy 2001 Girls | Eastern Elite Soccer Accademy | KY |
| MSC 01 Lady Strikers Premier | Murfreesboro Soccer Club | TN |
| NSA Belles 19 | Northwest Soccer Academy | GA |
| WCSA 01/02G Composite Elite | Warren County SA | OH |