| CFC05G Hendersonville NC | Carolina Football Club | SC |
| Oldham Soccer Club 04G Thoroughbreds | Oldham Soccer Club | KY |
| RCUSC 05G | River City United Soccer Club | AL |
| Stones River FC 2005 | Stones River Futbol Club | TN |
| Tudela Futbol 05 Girls Premier | Tudela FC | IN |
| TUSC 05G Red | Tennessee United Soccer Club | TN |
| FC Alliance 05B ENPL | FC Alliance (TN) | TN |
| MSC Strikers 04B Premier | Murfreesboro Soccer Club | TN |
| Southern Premier Soccer 04 Boys | Southern Premier Soccer | TN |
| United FC - Furman 04Boys elite | United FC - Furman | SC |
| FC Alliance 04B TSL2 | FC Alliance (TN) | TN |
| PSA LANUS | Premier Soccer Academy (TN) | TN |
| Rc3 FC Bandits | Rc3 FC | TN |
| Redoubt Generals 04 | Redoubt Soccer | TN |
| MSC 03 Lady Strikers | Murfreesboro Soccer Club | TN |
| OCSA COASTAL CREW G | Onslow Classic Soccer Assn - (OCSA) | NC |
| Tudela 03/04 girls | Tudela FC | IN |
| USC 03 Girls Premier | United Soccer Club (AL) | AL |
| All-In FC TN 03's | All-In Futbol Club Tennessee | TN |
| FC Alliance 03 Boys ENPL | FC Alliance (TN) | TN |
| FC Alliance 04B ENPL | FC Alliance (TN) | TN |
| MFCY 03 Revolution | Nashville FC (TN) | TN |
| TSC 04B Showcase | Tennessee Soccer Club (TN) | TN |
| United FC - Furman 03B Elite | United FC - Furman | SC |
| CASA 02G Premier | CASA Pride | SC |
| FC Alliance 03G ECNL | FC Alliance (TN) | TN |
| GSA North 02G Premier | Gwinnett Soccer Academy (GSA) | GA |
| NKSA SparX U17 | Northern Kentucky Soccer Academy | KY |
| Commonwealth SC 2002 Girls Gold | Commonwealth Soccer Club | KY |
| FC Alliance 02G Red | FC Alliance (TN) | TN |
| LSC Lady Force 02G | Lakeway Soccer Club | TN |
| RCUSC Stars 02G | River City United Soccer Club | AL |
| DSC 02 Gold Premier | Discoveries Soccer Club | NC |
| FC Tippecanoe Freedom | FC Tippecanoe | OH |
| MSC Strikers 02B Premier | Murfreesboro Soccer Club | TN |
| United FC - Furman 02B Black | United FC - Furman | SC |
| Chattanooga FC 02 Elite | Chattanooga FC Academy | TN |
| FC Montgomery U-17 | FC Montgomery | AL |
| MFCY 02/03B Revolution | Nashville FC (TN) | TN |
| TUSC 02 Boys Red | Tennessee United Soccer Club | TN |
| CFC 01G Elite | Carolina Football Club | SC |
| Chicago Rush South 01 Premier | Chicago Rush | IL |
| MSC 01 Lady Strikers Premier | Murfreesboro Soccer Club | TN |
| Tampa Dynamo 18G Elite | Tampa Dynamo | FL |
| FC Alliance 02 Boys ENPL | FC Alliance (TN) | TN |
| Greeneville Galaxy HS Boys | Greeneville Soccer Association | TN |
| TSC 01B Showcase | Tennessee Soccer Club (TN) | TN |
| United FC - Furman 01B Black | United FC - Furman | SC |
| BUSC 00 Boys | Blount United Soccer Club | TN |
| Chattanooga FC 00/01 Elite Boys | Chattanooga FC Academy | TN |
| MSC Strikers 01B Elite | Murfreesboro Soccer Club | TN |
| NFC 01 Navy | Nashville FC (TN) | TN |
| FC Alliance 00G ECNL Composite | FC Alliance (TN) | TN |
| FC ALLIANCE 02G ECNL | FC Alliance (TN) | TN |
| FC Montgomery 2000 Girls | FC Montgomery | AL |
| MOBA 00 Girls | MOBA Soccer Academy | GA |
| SSA Northside 00G | SSA Northside | GA |
| SWVA RUSH 00G NERO | SWVA Rush | VA |
| FC Alliance 00/01 B ENPL | FC Alliance (TN) | TN |
| MSC Strikers 00/01 Premier | Murfreesboro Soccer Club | TN |
| Rc3 FC Bridge | Rc3 FC | TN |
| Stones River FC 2000 | Stones River Futbol Club | TN |
| FC Alliance 00 Boys TSL2 | FC Alliance (TN) | TN |
| FC Montgomery U19 | FC Montgomery | AL |
| MUFC 00/01 | McMinn United Football Club | TN |
| PSA RIVER PLATE | Premier Soccer Academy (TN) | TN |