| FC National 07/08 Boys | FC National (TN) | TN |
| Hoosier FC 2008B Elite Wolves NL | Hoosier Futbol Club | IN |
| Knoxville Reds 08 Celtic | Knoxville Reds FC | TN |
| MUSA Flyers 08B | Madison United SA-Flyers MUSA (KY) | KY |
| NSA 2009 Boys Academy Gray | Nationals Soccer Club | MI |
| USAI 2008B White Premier Brownsburg | United Soccer Alliance of Indiana | IN |
| Aris FC 07B Black | Aris FC | TN |
| BGFC Golden Lions Academy 07 Gold | BGFC | KY |
| BUSC Gold Hammers | Blount United Soccer Club | TN |
| HCSA UNITED GOLD | High Country Soccer Assn (HCSA) | NC |
| VA United Football Club | Virginia United FC | VA |
| YMCA Arsenal Rome 07 Boys | YMCA Arsenal Rome | GA |
| 06 Fleming | Fleming County Soccer | KY |
| Javanon 2006 Boys Black | Javanon Futbol Club (KY) | KY |
| MUSA Flyers | Madison United SA-Flyers MUSA (KY) | KY |
| NRU 06B Blue | Virginia United FC | VA |
| United FC - Furman 06B Elite | United FC - Furman | SC |
| YMCA Arsenal Rome 06 Boys Gray | YMCA Arsenal Rome | GA |
| BGFC Golden Lions Academy 05 Gold | BGFC | KY |
| Chattanooga Red Wolves 04/05 Red | Chattanooga Red Wolves Academy | TN |
| Cincinnati West CW Inferno | Cincinnati West SC (CWSC) | OH |
| Tri-Cities United 04/05 Boys Premier | Tri-Cities United Soccer Club | TN |
| BGFC Golden Lions Academy 05 Black | BGFC | KY |
| Knoxville Reds FC 05 Lions | Knoxville Reds FC | TN |
| TUSC 2005 Boys Red | Tennessee United Soccer Club | TN |
| VHSC Steamers 04/05 Black | Vestavia Hills Soccer Club | AL |
| All-In FC TN HS Boys | All-In Futbol Club Tennessee | TN |
| FC Sudamerica 05B GPL | FC Sudamerica | GA |
| Rossoneri 05 Boys | Derby City Rossoneri | KY |
| Tri-Cities United 06 Boys Premier | Tri-Cities United Soccer Club | TN |